How we obtained improved variants of an enzyme (through in silico modeling) without structural data

In August 2020 we started a collaboration with a customer from the Biotech sector who wanted to increase the activity and specificity of an enzyme for a target substrate.

However, we were facing an outstanding situation. Our customer’s project enzyme had:

  • No known crystal structure
  • Very few known sequences similar to the wild-type, hindering the use of sequence-based tools
  • Unavailability of appropriate templates to build a good quality homology model

Download the PDF to find out how we approached this challenge.

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