Mapping distal mutations allowed us to extend the enzyme’s operational life by 40-fold, a critical factor for long-term industrial cycles.
Improving dioxygenases for sustainable vanillin production
Breaking IP barriers in API production
The enzyme engineering campaign yielded a lead candidate that significantly surpassed existing benchmarks. Experimental validation confirmed that Zymvol’s top hit delivered 10x higher activity than the leading patented enzyme, as well as 99% e.e.
Delivering a 17x Specificity Boost in 6 Months
After five intensive rounds of directed evolution, a leading biotech company had achieved an 8x increase in enzyme activity and a 12x increase in enzyme specificity. However, they had reached a plateau.
Improving Artificial Enzyme Performance with Distal Mutations
By screening a small number of mutant enzymes, the team discovered two individual single distant mutations —located more than 11 Å from the active site— that significantly improved the enzyme’s properties.
Simpler oxygenated product synthesis by altering enzyme specificity
In only one month, we went from a highly specific bacterial peroxygenase to a versatile enzyme capable of hydroxylating both indole and thioanisole, two substrates it previously could not act on.
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Create new products and processes, adapt existing ones or develop completely new biochemistry. Zymvol is here to guide you in any stage of your journey.
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