After five intensive rounds of directed evolution, a leading biotech company had achieved an 8x increase in enzyme activity and a 12x increase in enzyme specificity. However, they had reached a plateau.
Delivering a 17x Specificity Boost in 6 Months
Improving Artificial Enzyme Performance with Distal Mutations
By screening a small number of mutant enzymes, the team discovered two individual single distant mutations —located more than 11 Å from the active site— that significantly improved the enzyme’s properties.
Simpler oxygenated product synthesis by altering enzyme specificity
In only one month, we went from a highly specific bacterial peroxygenase to a versatile enzyme capable of hydroxylating both indole and thioanisole, two substrates it previously could not act on.
Start your Evolution
Create new products and processes, adapt existing ones or develop completely new biochemistry. Zymvol is here to guide you in any stage of your journey.
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